Endometrial Stem cell count induced by Immuno-cyto labeling CD 105 and CD 90 marker staining in the healthy women individuals
Department of Biology, College of Education for women, University of Kirkuk.
Research Article
Open Access Research Journal of Biology and Pharmacy, 2024, 12(02), 016-023.
Article DOI: 10.53022/oarjbp.2024.12.2.0041
Publication history:
Received on 02 November 2024; revised on 16 December 2024; accepted on 18 December 2024
Abstract:
Background: The current study was aimed to detect and localized of human endometrium stem cells at different age, through using CD105 and CD90 as stem cell markers.
Materials and methods: Fifty specimens of female adult endometrium from different age groups, including 20, 30, 40, 50, and 60, years were prepared for histological technique. Samples of uterine tissue were fixed in 10% buffer formalin and paraffinized as blocks, which were used for immunohistochemical studies localized and count the number of stem cells in endometrium of adult women. An immunohistochemical kit supplied by Pathn Situ PolyExel HPR/DAB detection system is used in present study.
Results: Expression of CD105 and CD90 given a clear result for the detection of endometrial stem cells (EnSCs). Results of the present study showed a highly expression count was found with CD90 more than with CD105. The localized expression was appeared in basalis stroma more than the functionalis. The means of CD90 expression in basalis were 45.20 ±8.67, 37.00±5.69, 34.60±4.88, 31.80±4.38 and 30.60±3.78 in I, II, III, IV and V age groups respectively. While in functionalis were 38.60±9.21, 37.40±7.73, 28.40±4.04, 25.00±2.92, 22.00±2.12 in I, II, III, IV, V age groups. The results of expression values showed inverse relationship were decreasing with aged, and the mean cellular count of CD105 expression within basalis stroma was 44.20±8.14, 40.20±9.98, 34.20±3.35, 31.40±4.34, 30.40±5.36 in I, II, III, IV, V age groups, otherwise the mean of CD105 expression in functionalis were 36.40±6.43, 35.40±9.84, 32.20±4.76, 26.20±2.59, 24.00±3.94 of the same age groups. The results of CD 105 and CD 90 cellular markers in both basalis and functionalis stroma of endometrium in group I showed a significant difference when compared with other groups on level P ≤ 0.05.
Conclusion: it was demonstrated in present study show the stem cells were decreasing in endometrium stroma gradually with increasing age. Expression rate of CD90 marker was more than CD105 on the other hand these markers showed a highly expression in basalis stroma than functionalis stroma
Keywords:
CD105; CD90 cell markers; Immunohistochemistry; Uterine endometrial
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