Metabolite profile analysis of methanol extract of belulang grass (Eleusine indica (L.) GAERTN) leaves by gas chromatography-mass spectrophotometry (GC-MS) method
1 Master of Biology Study Program, Faculty of Mathematics and Natural Sciences, Sriwijaya University, Indonesia.
2 Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University, Indonesia.
3 Chemistry Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University, Indonesia.
Research Article
Open Access Research Journal of Biology and Pharmacy, 2024, 12(02), 001–008.
Article DOI: 10.53022/oarjbp.2024.12.2.0037
Publication history:
Received on 16 September 2024; revised on 21 October 2024; accepted on 24 October 2024
Abstract:
Leaf of belulang grass (Eleusine indica (L.) Gaertn) is one of the wild plants that is used as herbal medicine by the community because of its many health benefits for the treatment of certain diseases such as diabetes, high blood pressure, and cholesterol. A materil is said to have potential as a medicine because the composition of the chemical compounds contained in a plant is not always the same and requires further testing. Differences in chemical compound content can be influenced by environmental factors and plant metabolism. The aim of this research is to analyze the metabolite profile and abundance of metabolite compounds using the GC-MS instrument analysis approach. Sampling was conducted in Sidomulyo Village, Air Kumbang District, Banyuasin Regency, South Sumatra. Samples of belulang grass leaves obtained were dried, pulverized, and extracted with methanol solvent. The methanol extract was analyzed using GC-MS. The results showed that the highest 20 compound peaks were obtained based on the relative area percent of the 69 compounds obtained with an abundance of compounds amounting to 80.86%. At the highest peak, the compounds n-hexadecanoic acid, I-(+)-ascorbic acid 2, 6- dihexadecanoate, and pentadecanoic acid were found with a percent area of 31.25%.
Keywords:
Leaf of Belulang Grass (Eleusine indica (L) Gaertn); GC-MS; Metabolite Profile; Sidomulyo Village; Herbal Medicine
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