Total Laboratory Automation (TLA) is the future of laboratory diagnostics due to its efficiency, reproducibility, better turnaround time (TATs), precision, sensitivity, and specificity. Microbiology is generally considered a human dependent field and still, most of the microbiology world is confused with TLA implementation. Two better-claimed technologies BD Kiestra InoqulA and Copan WASP have emerged as a well satisfactory solution of microbiology automation in the last decade. Here we design a practical approach and reviewed all studies of BD Kiestra InoqulA and Copan WASP, assessed microbiology samples in a healthcare setting.